For Sabine absorption coefficient measurements in reverberation rooms, it is preferable to excite as many room modes as possible. But the number of excited room modes is significantly affected by many factors, among which one dominating factor is the loudspeaker location particularly at frequencies below the Schroeder frequency. In turn, this could significantly influence the statistical absorption coefficient obtained according to ISO 354. Therefore, this study aims to investigate the impact of loudspeaker configurations on the excitation of room modes. Frequency response functions are measured using a broad band steady state noise signal using four sound sources at various locations, and the numbers of excited room modes in the one-third octave bands are quantified and compared against those by Greens function simulations. A procedure for determining favorable loudspeaker positions based on the excited room modes is proposed, which can be a useful input to the working group of the ISO 354 standard.