In this study, the general sound environment characteristics of open-plan office (OPO) were investigated, and just noticeable difference (JND) of sound pressure level of speech at a distance of 4 m (Lp,A,S,4m) suggested in ISO 3382-3 was suggested. First, in order to understand the sound environment characteristics of OPO, one minute sound sources recorded in 8 offices were collected and physical and psychological acoustic characteristics were analyzed. A total of 30 office workers were subject to subjective evaluation on 8 sound sources, and they were asked to respond to questionnaires related to annoyance, work satisfaction, and speech privacy. Next, to investigate the JND, two computer simulation models identical to those of the actual OPO were implemented, and sound sources each having six different Lp,A,S,4m values were generated through the change of the sound absorption coefficient of the interior finish. The JND of Lp,A,S,4m was presented by performing paired comparison for the same subjects. It is expected that the JND of Lp,A,S,4m proposed in this study can be used for the sound environment rating of OPO.